Gene description for PTPRC
Gene name	protein tyrosine phosphatase receptor type C
Gene symbol	PTPRC
Other names/aliases	B220 CD45 CD45R GP180 IMD105 L-CA LCA LY5 T200
Species	Homo sapiens
Experiment description of studies that identified PTPRC in B cells
1 Experiment ID 11 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] PubMed ID 12519789    Organism Homo sapiens Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. Authors Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mös W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W Journal name JBC Publication year 2003 Sample B cells Sample name RN (HLA-DR15+) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting Thin layer chromatography High performance liquid chromatography EV-TRACK -
2 Experiment ID 79 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
3 Experiment ID 80 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
4 Experiment ID 81 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
5 Experiment ID 308 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry [MALDI TOF/TOF] Mass spectrometry [Q-TOF] PubMed ID 16342139    Organism Homo sapiens Experiment description Proteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization Authors Miguet L, Pacaud K, Felden C, Hugel B, Martinez MC, Freyssinet JM, Herbrecht R, Potier N, van Dorsselaer A, Mauvieux L Journal name Proteomics Publication year 2006 Sample B cells Sample name B cells - Chronic B cell lymphoid patient Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [MALDI TOF/TOF] Mass spectrometry [QTOF] EV-TRACK -
6 Experiment ID 395 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 19413345    Organism Homo sapiens Experiment description Proteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis. Authors Miguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S. Journal name J Proteome Res Publication year 2009 Sample B cells Sample name Patients of chronic lymphocytic leukemia-B cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
7 Experiment ID 396 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 19413345    Organism Homo sapiens Experiment description Proteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis. Authors Miguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S. Journal name J Proteome Res Publication year 2009 Sample B cells Sample name Patients of mantle cell lymphoma-B cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
8 Experiment ID 397 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 19413345    Organism Homo sapiens Experiment description Proteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis. Authors Miguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S. Journal name J Proteome Res Publication year 2009 Sample B cells Sample name Patients of small cell lymphoma-B cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
9 Experiment ID 534 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name EBV- and KSHV-infected B cells (JSC-1, BC1) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
10 Experiment ID 535 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name EBV-infected B cells (#1, HLJ, IM9, CP) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
11 Experiment ID 536 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name KSHV-infected B cell (JC, BC3, BCP1, BCBL1) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
12 Experiment ID 537 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name Normal B cells (BJAB) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%